Journal: BMC Biotechnology

Article Title: Low density DNA microarray for detection of most frequent TP53 missense point mutations

doi: 10.1186/1472-6750-5-8

Figure Lengend Snippet: Detection of TP53 silent and missense base substitutions using DNA sequencing versus DNA microarray hybridization.

Article Snippet: In the present work we designed a novel small format DNA chip (genosensor array) to search for nine point mutations in codons 248, 249 and 273 of the TP53 gene.

Techniques: DNA Sequencing, Microarray, Hybridization, Sequencing, Mutagenesis

Journal: BMC Biotechnology

Article Title: Low density DNA microarray for detection of most frequent TP53 missense point mutations

doi: 10.1186/1472-6750-5-8

Figure Lengend Snippet: Layout of the TP53 low density DNA microarray . (A): Names and alignment of stacking oligonucleotides and probes to their respective synthetic wild type target sequence. Bold letters correspond to the nucleotide change in DNA sequence due to the point mutations. (B): Layout of the probe array on the glass slide. The probes were applied to the slide in triplicate as depicted at the top. The numbers correspond to the probes in Table 1.

Article Snippet: In the present work we designed a novel small format DNA chip (genosensor array) to search for nine point mutations in codons 248, 249 and 273 of the TP53 gene.

Techniques: Microarray, Sequencing

Journal: PLoS ONE

Article Title: Hiwi Mediated Tumorigenesis Is Associated with DNA Hypermethylation

doi: 10.1371/journal.pone.0033711

Figure Lengend Snippet: (A) Gene expression profiles of sh-Hiwi MFH cells treated with 1 uM or 5 uM 5-azacytidine cluster with gene expression profiles of doxycycline-induced sh-Hiwi MFH cells, using the whole gene set from both treatments. (B) Meth27 Illumina array of methylation of 27000 CpG site changes in sh-Hiwi MFH cells uninduced (x-axis) or induced with doxycycline for 7 days (y-axis). Graph shows all CpG islands and reveals no global shift in CpG methylation.

Article Snippet: These data suggest that CpG methylation status of genes (at least the 14,495 on the Illumina Meth27 array) are not affected; and thus that the overall change in global DNA methylation observed may be accounted by either DNA-methylation of repetitive elements (non-gene regions) or non-CpG gene methylation.

Techniques: Expressing, Methylation, CpG Methylation Assay